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Advanced Analytical Centre
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Analytical Facilities
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All Instruments
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General sample requirements
For SEM imaging there are a number of considerations when preparing sample.
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Materials need to be able to withstand the vacuum inside the specimen chamber. Thus samples need to be dry and have sufficient strength/rigidity. Particular care need be taken with biological samples in this regard (see additional notes).
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Samples need to be electrically conductive in order to prevent the negatively charged electrons from building up on the sample. Where material is not conductive a fine layer of a conductive medium is first coated on the surface (typically gold).
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Sample size should be kept to a minimum especially where coating is required.
AAC staff can help/advise on all aspects of sample preparation.